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2 edition of Investigations into the expression of catabolic Gene derived from Pseudomonas and Azotobacter. found in the catalog.

Investigations into the expression of catabolic Gene derived from Pseudomonas and Azotobacter.

Homa Koosha

Investigations into the expression of catabolic Gene derived from Pseudomonas and Azotobacter.

by Homa Koosha

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Published by Brunel University in Uxbridge .
Written in English


Edition Notes

ContributionsBrunel University. Department of Biology and Biochemistry.
The Physical Object
Pagination217p. :
Number of Pages217
ID Numbers
Open LibraryOL19720482M

The rol (designated for r esorcin ol) gene cluster rolRHMD is involved in resorcinol catabolism in Corynebacterium glutamicum, and RolR is the TetR-type this study, we investigated how RolR regulated the transcription of the rol genes in C. transcription start sites and promoters of rolR and rolHMD were identified. Quantitative reverse transcription-PCR and promoter. Various resorcinol compounds are produced in nature as secondary plant products ().Early studies indicated that resorcinol was degraded via three different pathways in bacteria: In Azotobacter vinelandii, resorcinol was converted into pyrogallol, and subsequently the aromatic ring was cleaved by a pyrogallol 1,2-dioxygenase (). Pseudomonas putida apparently adopted two different pathways.

The nucleotide sequence of the biphenyl catabolic transposon Tn has been completed and analyzed. It confirmed that the element has a mosaic structure made of several building blocks. In addition to previously identified genes coding for a tyrosine recombinase related to phage integrases and for biphenyl degradation enzymes very similar to those of Achromobacter georgiopolitanum KKS, . Micro RNAs effectively silence gene expression since the defective gene's protein product will not be produced. Please refer to section in the textbook for additional information. The study of cell phenotypes and all of the proteins made through the expression of .

Abstract. Soil and water bacteria in general, and members of the genus Pseudomonas in particular, exhibit a fascinating wealth of exotic properties not found in commensal and parasitic bacteria, such as Escherichia are, for example, able to degrade and use as sources of carbon and energy a wide range of organic compounds, including some that are quite noxious and otherwise biocidal. 2 days ago  In bacteria, indole alkaloids are mostly derived from tryptophan or its direct precursor indole, which itself is formed from chorismate through anthranilate and indoleglycerol-phosphate in microorganisms. Since the final step of tryptophan biosynthesis is reversible, free indole can also be formed in this catabolic process.


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Investigations into the expression of catabolic Gene derived from Pseudomonas and Azotobacter by Homa Koosha Download PDF EPUB FB2

Investigations into the expression of catabolic gene derived from pseudomonas and azotobacter. (Thesis) ' ' Koosha H Publisher: Brunel University [] Metadata Source: The British Library Type: Thesis. Abstract. No abstract provided. Menu. Formats.

Abstract. EThOS. About. Investigations into the expression of catabolic gene derived from pseudomonas and azotobacter. Author: Koosha, Homa. ISNI: Awarding Body: Brunel University Gene regulation Share: Terms and. Topics: 06V - Genetics, cytology, molecular biology, 06M - Microbiology, 06B - Bioengineering, biomedical engineering, biotechnology, biochemical engineering, Investigations into the expression of catabolic gene derived from pseudomonas and azotobacter [ Gene regulation]Author: H Koosha.

We used a P. aeruginosa microarray to compare gene expression in planktonic and biofilm cells array contained 5, of the predicted Cited by: Azotobacter vinelandii strain degrades benzoate via the meta-cleavage pathway.

In a genomic library derived from this organism a clone was obtained which carried and expressed the gene for the Author: Henry Keil. Pseudomonas aeruginosa is capable of sensing and responding to different environmental stimuli, carbon and nitrogen sources, and also to several stresses such as starvation, osmosis, oxidation state, temperature change, and desiccation.

This adaptability, in comparison with other bacterial species, has been suggested to be attributable to the high proportion of putative catabolic. Ogawa, N., and Miyashita, K.,The chlorocatechol-catabolic transposon Tn of Alcaligenes eutrophus NH9, carrying a gene cluster highly homologous to that in the 1,2,4-trichlorobenzene-degrading bacterium Pseudomonas sp.

strain P51, confers the ability to grow on 3-chlorobenzoate. pWW is a cointegrate between RP4 and the catabolic plasmid pWW53 from Pseudomonas putida MT53, which contains 36 kbp of pWW53 DNA inserted close to the oriV gene.

Pseudomonas is a widespread bacterial genus embracing a vast number of species. Various genosystematic methods are used to identify Pseudomonas and differentiate these bacteria from species of the same genus and species of other genera.

Ability to degrade and produce a whole spectrum of compounds makes these species perspective in industrial applications. A high-level expression cassette has been constructed from a TOL plasmid derived fromPseudomonas putida carrying allcis- andtrans-acting regulatory elements necessary for transcriptional gene activation in the presence of aromatic hydrocarbons such as n DNA can be inserted at unique KpnI, SacI, andEcoRI sites 7, 13, and 15 nucleotides downstream of a.

The gene encoding the α subunit, pcaG, has been reported to be located downstream from pcaH, the gene for the β subunit, for Agrobacterium tumefaciens, B. cepacia, Acinetobacter spp., and Pseudomonas spp.

(representatives of the α- β- and γ-Proteobacteria), as well as for Rhodococcus opacus, a gram-positive nocardioform actinomycete ( Polaromonas naphthalenivorans CJ2, found to be responsible for the degradation of naphthalene in situ at a coal tar waste-contaminated site (C.-O.

Jeon et al., Proc. Natl. Acad. Sci. USA Protocatechuate 3,4-dioxygenase (EC [][1]) catalyzes the ring cleavage step in the catabolism of aromatic compounds through the protocatechuate branch of the β-ketoadipate pathway.

A protocatechuate 3,4-dioxygenase was purified from Streptomyces sp. strain grown in p -hydroxybenzoate, and the N-terminal sequences of the β- and α-subunits were obtained. PCR. A species of bacteria whose spores vary from round to elongate.

It is a common soil saprophyte. | Explore the latest full-text research PDFs, articles, conference papers, preprints and more on. Studies have reported that the alginate biosynthesis consists of a complex series of gene clusters in Pseudomonas aeruginosa (single operon) and Azotobacter vinelandii (3 operons) cells that controlled both EPS molecules production and its extracellular secretion (Czaczyk and Myszka, ; Kumar et al., ; Whitney and Howell, ).

Gene transcription assays. Reverse transcription-quantitative PCR (RT-qPCR) was used to assess relative gene expression. Transcripts diagnostic of the benzoyl-CoA pathway (boxA) and the protocatechuate branch of the β-ketoadipate pathway (pcaH) were measured and normalized to the expression of three reference genes (alaS, map, and rpoC).Primers were designed for each of these.

Azotobacter vinelandii gene sequence, which has very recently been deposited in the GenBank database, and that it has 64% identity to the Pseudomonas aeruginosa gene sequence but that it has.

Explain the regulation of gene expression in bacteria by induction, repression, and catabolic repression. A cell's genetic machinery and its metabolic machinery are integrated and interdependent. Regulation of protein synthesis is important to the cell's energy economy. Gene regulation studies in pseudomonad bacteria are mainly restricted to Pseudomonas aeruginosa and Pseudomonas putida.

Constitutive promoters exhibit DNA sequences similar to the σ dependent constitutive promoters of Escherichia coli. The TOL meta-cleavage pathway operon promoter and the nah operon promoters are the best characterized σ dependent promoters, which exhibit regions.

Van Aken, in Comprehensive Biotechnology (Second Edition), Transgenic plants expressing phase I enzymes of the green liver model.

In a pioneering study, Doty et al. [15] transformed tobacco plants expressing a human cytochrome P 2E1 (CYP2E1), known to be involved in the oxidation of a wide range of toxic halogenated compounds, including TCE, CT, and chloroform.

Closer examination revealed that the two genes flanking this RGP in the other strains, were fused in C into a single ORF (fusion of PACG_ and PACG_) creating a chimeric gene encoding a putative residue protein that consists of the N-terminal amino acids derived from the coding sequence of the right anchor (PA in the.Initial investigations suggested that the nah gene was highly conserved among PAH degrading bacteria, but as genes encoding PAH metabolism in a wider range of bacteria were characterized it became clear that the nah-like genes from pseudomonads provided a limited view of the gene diversity involved in the initial oxidation of PAHs, even amongst.Closer examination revealed that the two genes flanking this RGP in the other strains, were fused in C into a single ORF (fusion of PACG_ and PACG_) creating a chimeric gene encoding a putative residue protein that consists of the N-terminal amino acids derived from the coding sequence of the right anchor (PA in the.